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The 2nd response cycle in NO synthesis includes O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to produce ferric enzyme, NO, citrulline, and water as items (Fig. 9) - UV/Vis. As attracted Fig - https://linktr.ee/olisclarity1. 9, the reaction might also involve electron transfer from H4B to the Fe, IIO2 intermediate
l9 As displayed in Fig. 9, this could be due to the fact that the H4B radical acts as an electron sink in a later point in the reaction, so regarding enable NOS to, Fig. 9. A design for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen see this binds to the ferrous protein to form the Fe, IIO2 types, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.